Devices and methods for eliminating termite colonies

ABSTRACT

This invention relates to devices, kits, and methods for eliminating termite colonies. The kits, devices, and methods employ a termiticidal bait matrix containing
         a) a termiticide selected such that the termiticide causes death to about 50 to about 100% of termites within about 24 to about 84 days after the termites begin to ingest the termiticide or the bait matrix comprising the termiticide,   b) a cellulose containing material, and   c) water.
 
The termiticidal bait matrix can be used in a bait station installed in the ground. The kits are suitable to be used by consumers in their homes.

This is a Divisional of application Ser. No. 09/799,184 filed Mar. 5,2001 now U.S. Pat. No. 6,716,421.

FIELD OF THE INVENTION

This invention relates to devices and methods for eliminating termitecolonies. More particularly, this invention relates to bait matrices andbait stations for eliminating termite colonies and methods for theiruse.

BACKGROUND

Termites have a social hierarchy with several general adult forms:workers, soldiers, secondary reproductives, and a primary male (king)and primary female (egg-laying queen). Workers make up the largest partof the colony, and among their tasks is foraging for food for themembers of the colony. Workers are the colony members that cause damageto wood. Workers communicate information related to sources of food fromone termite to another by chemical odor (pheromones) communication andtouch (tactile) communication. Workers also carry food from its sourceback to the colony where it is shared with other colony members bytrophallaxis.

Quick kill of individual foraging workers does not affect the maincolony because it has no effect upon those hatching in the nest. Onlythe feeding termites are affected, those in the nest continue tomultiply and thus the infestation remains. However, if a “slow acting”termiticide is mixed with a food source desirable to termites, theforaging workers will communicate the location of the food source toother workers, and the foraging workers will also carry the termiticidecontaining food back to the nest to be shared. If sufficient termiticideis transported back into the nest, it is possible to eliminate theentire colony.

One problem in eliminating a termite colony is locating the colony.Various species of termites may have nests that are subterranean, withinthe structure of homes, or “aerial” (in trees, under roofs, etc.).Subterranean termites include Reticuliterme flavipes (Easternsubterranean termites), Reticulitermes hesperus, Reticulitermesvirginicus, Coptotermes formosans (Formosan termites), and Heterotermesaureus. Other types of termites include dry wood termites such asKalotermes minor, Kalotermes snyderi, Kalotermes schwarzi, andProcryptotermes hubbardi; damp-wood termites such as Prorhinotermessimplex; rotten-wood termites such as Zootermopsis angusticollis andZootermopsis nevadensis; powder-post termites such as Cryptotermesbrevis; and nasutiform termites such as Nasutitermes corniger.

Attempts have been made to address the problems discussed above by theuse of bait stations containing bait matrices. Some of the productscurrently available include FIRSTLINE® from FMC Corporation ofPhiladelphia, Pa., SLTBTERFUGE™ from American Cyanimid of Madison, N.J.,and SENTRICON® made by Dow AgroSciences of Indianapolis, Ind.SENTRICON®, for example, is a termite colony elimination systemconsisting of a bait station in the form of a plastic spike containerand a nontermiticidal bait matrix that is replaced with a termiticidalbait matrix containing a chemical insecticide when termites are detectedat the nontermiticidal bait matrix. The system is designed for insertionin and around buildings and structures. SENTRICON® is only available atconsiderable expense from a licensed pest control operator (“PCO”).Typically, the PCO will install a plurality of bait stations containingthe nontermiticidal bait matrix in the soil around the foundation of thestructure to be monitored and protected. The PCO contracts to visit thesite periodically after installation to check the bait stations forevidence of termite infestation or feeding on the bait matrix. Iftermite presence is detected in a bait station, the nontermiticidal baitmatrix is replaced with a termiticidal bait matrix (containing achemical insecticide). The PCO continues to visit the site periodicallyand replace spent termiticidal bait matrix until the colony iseliminated.

The chemical insecticide in SENTRICON® is hexaflumuron, a benzoylureacompound. Benzoylurea compounds (such as hexaflumuron, flufenoxuron,lufenuron, and dimilin) are chitin synthesis inhibitors, which disruptthe molting cycle of termites. However, chitin synthesis inhibitorssuffer from the drawback that they are too slow-acting. It can take 4months to 1 year to eliminate a termite colony using hexaflumuron inthis way.

SUMMARY OF THE INVENTION

This invention relates to devices and methods for detecting the presenceof termites and eliminating termite colonies. The devices and methodstypically employ cellulase inhibitors as termiticides. Cellulose (in theform of wood, paper, etc.) is a source of food for termites. Termitesrequire cellulases to digest the cellulose to glucose. Without wishingto be bound by theory, it is thought that cellulase inhibitors ingestedby a termite prevent one or more of the cellulases in the gut of thetermite from digesting cellulose, at least to some degree, e.g., adegree sufficient to kill the termite.

Further objects and advantages of this invention will be apparent fromthe following detailed description of the presently preferredembodiments which are illustrated schematically in the accompanyingdrawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a bait station according to this invention.

FIG. 2 is a bait station according to this invention.

FIG. 3 is a bait station according to this invention.

FIG. 4 a is a bait station according to this invention.

FIG. 4 b is a bait station according to this invention.

FIG. 5 is a device for installing a bait station according to thisinvention.

FIG. 6 a is a closed view of a device for installing a bait stationaccording to this invention.

FIG. 6 b is an open view of a device for installing a bait stationaccording to this invention.

FIG. 6 c is a cross section of a device for installing a bait stationaccording to this invention.

FIG. 7 is a probe for use in a kit according to this invention.

FIG. 8 is a kit according to this invention.

DETAILED DESCRIPTION OF THE INVENTION

Publications and patents are referred to throughout this disclosure. AllU.S. patents cited herein are hereby incorporated by reference.

All percentages, ratios, and proportions used herein are by weightunless otherwise specified.

Definitions

The following is a list of definitions for terms, as used herein.

“Attractant” means a compound that stimulates foraging termites tolocate and/or feed on compositions containing the compound over othercompositions and/or their regular food source.

“Cellulase” means a member of a class of enzymes that digest celluloseinto glucose. Cellulase is a general term for any of the enzymes thattogether make-up the cellulase complex.

“Cellulase complex” means a natural enzyme mix of multipleexoglucanases, endoglucanases, and β-glucosidase as well as otherenzymes produced by most organisms that produce cellulases.

“Cellulase inhibitor” means a compound, alone or in combination, thatprevents one or more of the cellulases in the gut of a termite fromdigesting cellulose, at least to some degree, e.g., a degree sufficientto kill the termite. Preferred cellulase inhibitors are specific tocellulase, i.e., they do not inhibit or change the action of manyproteins other than cellulases. More preferred cellulase inhibitors donot inhibit or change the action of proteins found in animals other thantermites, particularly humans. Most preferred cellulase inhibitors, forpurposes of this invention, do not inhibit or change the action of anyproteins other than cellulases.

“Feeding stimulant” means a compound that increases the amount thattermites eat of compositions containing the compound over othercompositions and/or their regular food source.

“Heteroatom” means an atom other than carbon e.g., in the ring of acyclic group or the chain of a substituted hydrocarbon group.Preferably, heteroatoms are selected from the group consisting ofsulfur, phosphorous, nitrogen and oxygen atoms. Groups containing morethan one heteroatom may contain different heteroatoms.

“Hydrocarbon group” means a chain of carbon atoms, preferably about 1 toabout 6 carbon atoms, more preferably about 1 to about 3 carbon atoms.Hydrocarbon groups may have a linear or branched chain structure.Preferred hydrocarbon groups are saturated. Unsaturated hydrocarbongroups have one or more double bonds, one or more triple bonds, orcombinations thereof. Hydrocarbon groups may be substituted orunsubstituted.

“Substituted” means one or more hydrogen atoms bonded to carbon atoms inthe chain of a hydrocarbon group or the ring of a cyclic group have beenreplaced with other substituents and/or one or more carbon atoms in thechain of a hydrocarbon group or the ring of a cyclic group have beenreplaced with one or more heteroatoms.

“Trophallaxis” means transfer of gut content or food from a termite toother colony members.

Bait Matrix

This invention relates to bait matrices. The bait matrices can betermiticidal or nontermiticidal. The termiticidal bait matrices can beused to eliminate a termite colony. The nontermiticidal bait matricescan be used to monitor for the presence of termites.

The termiticidal bait matrix comprises: a) a termiticide, b) a cellulosecontaining material, and c) water. The termiticidal bait matrix mayfurther comprise d) one or more optional ingredients.

Ingredient a) is a termiticide. The termiticide is typically selectedsuch that it causes death to about 50 to about 100% of termites withinabout 24 to about 84 days after the termites begin to ingest thetermiticide or a bait matrix comprising the termiticide. Preferably, thetermiticide causes death to about 50 to about 100% of termites withinabout 38 to about 70 days after the termites begin to ingest thetermiticide or a bait matrix comprising the termiticide.

Suitable termiticides in this invention include compounds of Formula I,below:

In formula I, R¹ is selected from the group consisting of a bond, ahydrocarbon group, O, and NR¹⁴R¹⁵. R¹ is preferably a hydrocarbon grouphaving 1 to 6 carbon atoms, more preferably 1 to 3 carbon atoms. R¹ ispreferably an unsubstituted hydrocarbon group. R¹⁴ and R¹⁵ are eachindependently selected from the group consisting of a hydrogen atom anda hydrocarbon group.

Each R² is independently selected from the group consisting of CH, acarbon atom, and a heteroatom. Preferably, the heteroatom is nitrogen.More preferably, R² is CH.

R³, R⁴, R⁵, and R⁶ are each independently selected from the groupconsisting of hydrogen atoms and hydroxyl groups. When R⁸ and R⁷ areoxygen atoms and R⁹ and R¹⁰ are hydrogen atoms, then R³, R⁴, R⁵, and R⁶may alternatively be hydrocarbon groups of 1 to 6 carbon atoms.

R⁷ and R⁸ are each independently selected from the group consisting ofhydrogen, oxygen, and nitrogen atoms. Preferably, R⁷ and R⁸ are eachindependently selected from the group consisting of oxygen and nitrogenatoms.

R⁹ and R¹⁰ are each independently selected from the group consisting ofnil (i.e., when R⁷ and/or R⁸ are hydrogen atoms), hydrocarbon groups of1 to 6 carbon atoms, alkyl esters of 1 to 6 carbon atoms, and amides.Preferably, R⁷ and R⁸ are oxygen atoms and R⁹ and R¹⁰ are hydrogenatoms.

R¹¹ and R¹² are each independently selected from the group consisting ofsaturated, unsaturated, and aromatic groups.

Examples of compounds of Formula I are shown below in Table I.

In Table I, Ac represents an acyl group.

Suitable termiticides for use in this invention also include compoundsof Formula II, below:

wherein each R¹⁶ is independently selected from the group consisting ofan oxygen atom and CH₂. R¹⁶ is preferably an oxygen atom.

each R¹⁷ is independently selected from the group consisting of C═O andCR²¹(R²²), wherein R²¹ and R²² are each independently selected from thegroup consisting of a hydrogen atom, OH, a halogen atom, CR²⁸R²³ ₂, andOR²⁴, wherein R²⁴ is an aromatic group that may optionally besubstituted with one or more groups such as NO₂. Each R²³ isindependently selected from the group consisting of a halogen atom, ahydrogen atom, a hydrocarbon group, an aromatic group, and an acylgroup; with the proviso that at least one R²³ is a halogen atom. Thepreferred halogen atom for R²³ is fluorine. The hydrocarbon group andthe aromatic group for R²³ may optionally be substituted. R²⁸ isselected from the group consisting of R²³ and groups of the formulae:

Each R¹⁸ is independently selected from the group consisting of ahydrogen atom, a halogen atom, and OH. The preferred halogen atom forR¹⁸ is fluorine.

Each R¹⁹ and each R²⁰ are independently selected from the groupconsisting of a hydrogen atom, and a group of the formula:

with the proviso that R¹⁹ and R²⁰ are not both the group of the formula

Preferably, R¹⁹ is a hydrogen atom.

Examples of compounds of Formula II are shown below in Table II.

In Table II, each R²⁵ and each R²⁶ is independently selected from thegroup consisting of a hydrogen atom and a halogen atom, preferablyfluorine. R²⁷ is a 4-glucopyranosyl group.

Suitable termiticides for use in this invention also include imidazolecompounds. Suitable imidazole compounds are shown below in Table III.

Suitable termiticides for use in this invention also include thecompounds shown below in Table IV.

Other suitable termiticides include n-bromyl succinimide,phenyl-beta-D-glycoside, 4,4′-isopropylidenedicyclohexanol,2,5,6-trimethylbenzylimidazole, hexachloropalladate compounds,polyphenols, and combinations thereof. Suitable hexachloropalladatecompounds include ammonium hexachloropalladate, sodiumhexachloropalladate, and combinations thereof. Suitable polyphenolsinclude Aspidosperma quebracho-blanco extract, Cucurbitaceae fruit &green tea extract, and combinations thereof.

The termiticide of this invention can be a single termiticide or acombination of two or more termiticides described above.

The termiticide used in this invention typically comprises a cellulaseinhibitor. Termites require digestive cellulases to hydrolyze thecellulose in the wood they ingest as food. The cellulases hydrolyzecellulose to glucose. Without wishing to be bound by theory, it isthought that cellulase inhibitors ingested by a termite prevent one ormore of the cellulases in the gut of the termite from digestingcellulose, at least to some degree, e.g., a degree sufficient to killthe termite. Suitable cellulase inhibitors include β-glucosidaseinhibitors, endoglucanase inhibitors, exoglucanase inhibitors, andcombinations thereof.

Preferred cellulase inhibitors are compounds specific to cellulase,i.e., they do not inhibit or change the action of many proteins otherthan cellulases. More preferred cellulase inhibitors are compounds thatdo not inhibit or change the action of proteins found in animals otherthan termites, particularly humans. Most preferred cellulase inhibitors,for purposes of this invention, are compounds that do not inhibit orchange the action of any proteins other than cellulases. Compoundsspecific to cellulase are preferred for use as the cellulase inhibitorsof this invention. Without wishing to be bound by theory, it is thoughtthat compounds specific to cellulases that do not inhibit or change theaction of other proteins will be safe (nontoxic) to humans and othermammals. One skilled in the art will recognize that while compoundsspecific to cellulase are preferred for use in this invention, they arenot limiting. Other cellulase inhibitors, which are not necessarilyspecific to cellulase, are also suitable to use in this invention.

Cellulase is a general term for any of the enzymes that together make-upthe cellulase complex. (Most organisms that produce cellulases produce acellulase complex consisting of a natural enzyme mix of multipleexoglucanases, endoglucanases, and β-glucosidase as well as otherenzymes.) Whether a compound is a cellulase inhibitor can be determinedwithout undue experimentation using methods known in the art. Manycellulase enzyme activity assays are available using a variety ofsubstrates (see for example, Methods in Enzymology, Volume 160, BiomassPart A, Cellulose and hemicellulose, ed. W. A. Wood & S. T. Kellogg,Academic Press, NY 1988).

To measure the enzyme activity of any one cellulase, a specific assayand substrate are selected for the specific cellulase of interest (e.g.,an appropriate substrate for β-glucosidase is p-nitrophenylβ-1,4-glucopyranoside). Other assays and substrates are more appropriateto measure enzyme activity of the cellulase complex (e.g., anappropriate substrate for the cellulase complex comprising a natural mixof exoglucanases, endoglucanases, and β-glucosidase is cellulose azure).

The enzyme assays are used to establish a rate for cellulase hydrolysisof the substrate in the absence of an inhibitor. Each potentialinhibitor is also mixed with the cellulase enzyme and the same assay isperformed on the combination of the cellulase and the potentialinhibitor. Cellulase inhibition is measured as a reduction in rate ofhydrolysis of the substrate, compared to the enzyme rate withoutinhibitor.

Suitable cellulase inhibitors for use in this invention include, but arenot limited to, the compounds shown below in Table V; compounds havingthe following CAS Numbers 10192-62-8, 19168-23-1, 85803436, 6222058-0,21393-76-1; and combinations thereof.

TABLE V Cellulase Inhibitors

In an alternative embodiment of the invention, the termiticide furthercomprises one or more slow acting toxicants. Suitable slow actingtoxicants include amidinohydroazones, amylase inhibitors,antiprotozoals, barium compounds, biological control agents, boroncompounds, chitin synthesis inhibitors, azadirachtin,fluooalkylsulfonamides, glucose antimetabolites, hexokinase andglucokinase inhibitors, iimidacloprid, juvenile hormones and juvenilehormone mimics and juvenile hormone analogs, macrolide antibiotics,metal compounds, combinations thereof, and others.

Suitable amidinohydroazones include avermectin, hydramethylnon,silafluofen, and combinations thereof.

Suitable amylase inhibitors include acarbose, cyclohexaamylose,trestatin, and combinations thereof.

Suitable antiprotozoals include toxins to termites' gut dwellingcellulase producing protozoa. Suitable antiprotozoals include5,7-diiodo-8-hydroxyquinoline (iodoquinol), di-(4-aminophenylsulfone)(dapsone), 1-methyl-4-diiodomethylsulfonylbenzene(diiodomethyl-para-tolylsulfone), and combinations thereof.

Suitable barium compounds include barium metaborate monohydrate.

Suitable biological control agents include fungi such as Metarhiziumanisopliae, Aspergillus flavus, and Beauveria bassiania; nematodes suchas Neoplectana carpocapsae; insect viruses; bacteria such as Bacillusthuringiensis and Serratia marcescens; and combinations thereof.

Suitable boron compounds include boric acid, disodium octaboratetetrahydrate, zinc borate, ulexite, colemanite, calcium boride, andcombinations thereof.

Suitable chitin synthesis inhibitors include hexaflumuron, flufenoxuron,lufenuron, and diflubenzuron (Dimilin), and combinations thereof.

Suitable fluoroalkylsulfonamides include sulfluramid.

Suitable glucose antimetabolites include 5-thio-D-glucose;2-deoxy-D-glucose; nojirimycin; nojirimycin bisulfide;1-deoxynojirimycin; and p-nitrophenyl-α-D-glucoside in mono-, di-, orpolymer form, and combinations thereof.

Suitable hexokinase and glucokinase inhibitors include lauric acid,myristic acid, glucosamine, 6-amino-6-deoxy-D-glucose,N-acetylglucosamine, and combinations thereof.

Suitable juvenile hormones and juvenile hormone mimics or analogsinclude fenoxycarb, methoprene, hydroprene, triprene, furnesinic acidethyl and alkoxy derivatives, pyriproxyfen (Nylar), and combinationsthereof.

Suitable macrolide antibiotics include abamectin, milbemycin, spinosynA, spinosyn D, and combinations thereof.

Suitable metal compounds are molybdenum or tungsten compounds. Suitablemolybdenum compounds include MoO₃, H₂MoO₄, CaMoO₄, Na₂MoO₄, FeMoO₄, andcombinations thereof. Suitable tungsten compounds include Na₂WO₄.

Suitable slow acting toxicants are disclosed in U.S. Pat. No. 4,504,468;U.S. Pat. No. 4,636,798; U.S. Pat. No. 5,609,879; U.S. Pat. No.5,637,298; U.S. Pat. No. 5,695,776; U.S. Pat. No. 5,756,114; U.S. Pat.No. 5,778,5961; and U.S. Pat. No. 5,937,571 which are herebyincorporated by reference for the purpose of disclosing suitable slowacting toxicants.

Typically the amount of ingredient a) in the bait matrix is about 0.1 toabout 10%. However, the exact amount of ingredient a) in thetermiticidal bait matrix will depend on the specific component orcomponents in the termiticide, e.g., the potency of each component. Thetermiticides of this invention are preferably slow acting, i.e., they donot kill the termite immediately after ingestion. Without wishing to bebound by theory, it is thought that if foraging termites are killed tooquickly after they ingest the termiticide, they will not have time tomake repeated visits to the source of the termiticide and communicatethe source to other termites or to transport it back to the colony.

Ingredient b) is a cellulose containing material. Examples include wood,charred wood, decayed wood, sawdust, pulp, paper, cotton linter,cellulose ethers, and combinations thereof. Suitable types of woodinclude basswood, aspen, cottonwood, paper birch, soft maple, yellowpoplar, beech, pecan, hard maple, persimmon, pine, fir, spruce, andcombinations thereof. Suitable types of paper include 100% virgin paper,recycled paper, a combination of virgin and recycled paper, paperboard,cardboard, and combinations thereof. Paper may be bleached, typicallywith one or more solutions, e.g., aqueous solutions, of bleachingchemicals. Paper can optionally be textered or roughened and mayoptionally comprise a plurality of plies. Suitable cellulose ethersinclude methylcellulose, hydroxypropylmethylcellulose,hydroxybutylmethylcellulose (commercially available as METHOCEL® fromthe Dow Chemical Company), and combinations thereof. Typically theamount of ingredient b) in the bait matrix is about 5 to about 50%.

Ingredient c) is water. The amount of water in the bait matrix istypically about 5 to about 50%.

Ingredient d) is one or more optional ingredients selected from thegroup consisting of attractants, defoamers, dextrin, dispersing agents,extenders, feeding stimulants, flavors, grain powder, moisture adjustingmeans, nitrogen sources, nutrients, penetrants, stabilizers, sugars,surface active agents, suspending agents, synergists, vegetable oils,and combinations thereof. Typically the amount of ingredient d) in thebait matrix is about 1 to about 10%.

Suitable attractants for ingredient d) include pheromones,2-naphthalene-methanol, naphthalene, 2-phenoxyethanol, steroidderivatives, cow dung, camphor, thujene, linalol, cadinene, turpentineoil, borneol, methylanisol, cinnamyl alcohol, isosafrole, steroidderivatives, and combinations thereof.

Suitable nutrients for ingredient d) include agar or a mixture of agarand a wet gel.

Suitable moisture adjusting means for ingredient d) include humectants.

Suitable feeding stimulants for ingredient d) include ergosterol,fermented milk, p-hydroquinone, hydroxyphenyl-β-D-glycopyranoside,catechol, resorcinol, fluoroglucinol, 4-methoxyphenol,1,4-dimethoxybenzene, 4-phenoxyphenol, phenylhydroquinone, 4benzyloxyphenol, quinhydrone, and combinations thereof. Hydroquinone ispreferred.

Suitable syngergists for ingredient d) includebutoxyethoxyethoxymethylenedioxypropyltoluene, octachlorodipropyl ether,isobornylthiocyanato acetate, ethylhexylbicycloheptenedicarboxyimide,and combinations thereof.

Suitable stabilizers for ingredient d) include butylated hydroxyanisole,butylated hydroxytoluene, tocophereols, and combinations thereof.

Suitable nitrogen sources for ingredient d) include uric acid, aminoacids, peptides, proteins, and combinations thereof.

In an alternative embodiment of the invention, a nontermiticidal baitmatrix can be prepared. The nontermiticidal bait matrix comprisesingredient b) described above. The nontermiticidal bait matrixoptionally further comprises ingredients c) or d), or both, describedabove. Typically, the nontermiticidal bait matrix comprises ingredientsb) and c). The nontermiticidal bait matrix does not contain atermiticide.

The exact composition of the bait matrices described above, i.e.,amounts of each ingredient and selection of ingredients, may depend onthe type of termites being targeted. For example, different types oftermites may prefer different types of wood. Without wishing to be boundby theory, it is thought that hardness of the wood is one factorcontributing to termites' preferences. Different types of termites alsomay prefer different feeding stimulants. Therefore, the bait matrix canbe targeted to kill different types of termites by selection ofcellulose containing materials, feeding stimulants, termiticides, andoptional ingredients in the bait matrix.

The bait matrix may have a variety of forms. The bait matrix may be asolid or a fluid. The bait matrix can be, for example, a solid piece ofwood coated with or impregnated with ingredients a), c), and optionallyd). In one embodiment of the invention, the bait matrix is a solid b)cellulose containing material coated or impregnated with ingredients a),c) and optionally d), wherein the solid b) has one or more groovestherein. For example, the solid b) may be a rectangular block having aplurality of grooves cut along one or more sides of the rectangularblock. The length of each groove preferably extends from one end of therectangular block to the other end. Preferably, the width of each grooveis about 1/32 inch to about 1 inch, preferably about ⅛ inch. In thisembodiment of the invention, the bait matrix has a form similar to thatin U.S. Pat. No. 5,695,776, which is hereby incorporated by reference.

The bait matrix can be used alone or in conjunction with a bait station.When the bait matrix is used alone, it is preferably in the form of asolid. The bait matrix may be placed in or near a locus of potential orknown termite activity. In an alternative embodiment of the invention,the bait matrix is used in a bait station.

Bait Station

This invention further relates to bait stations and kits including baitmatrices and bait stations. A suitable bait station comprises a housingadapted to receive one or more of the bait matrices described above. Thehousing has at least one opening sized to permit termites to passthrough the opening so that termites can gain access to the bait matrixfrom a location outside the housing. Typically, the housing has aplurality of openings. The housing is adapted to be installed in or neara locus of potential or known termite activity.

In an alternative embodiment of the invention the bait station furthercomprises a lid removably attached to an open end of the housing. Thiswill allow for removal or replacement, or both, of the bait matrix whenit is exhausted or partially exhausted. The bait station may furthercomprise locking means attaching the lid to the housing, for example, toprevent children from opening the bait station while it is in use.

The bait station may further comprise a removable receptacle for holdingthe bait matrix inside the housing, wherein the receptacle has at leastone opening, typically a plurality of openings, sized to permit termitesto pass through the opening. The openings in the housing and thereceptacle are typically at least partially aligned to allow termitesaccess to the bait matrix when the receptacle is installed inside thehousing.

In one embodiment of the invention, wherein the bait station will beinstalled in-ground, the bait station has dimensions suitable forinstallation using manual digging implements.

The bait station may comprise any suitable materials of construction;for example polyvinyl chloride or a thermoplastic material that isopaque, translucent, or transparent. In one embodiment of the inventionat least part of the bait station, e.g., the lid, is at least partiallytransparent so that termite activity can be monitored without moving oropening the housing. In an alternative embodiment of the invention, atleast part of the bait station, e.g., the lid, is colored to match itssurroundings when the bait station is installed.

An example of a suitable bait station is shown in FIG. 1. The baitstation 100 comprises a housing 101 adapted to receive a bait matrix(not shown). The housing has openings 102 sized to permit termites topass through to gain access to the bait matrix from a location outsidethe housing 101. The housing 101 has a metal tip 103 on one end and astrike plate 104 on the other end. The metal tip 103 and strike plate104 allow the bait station 100 to be driven into the ground by poundingwith, for example, a hammer or mallet.

Another bait station is shown in FIG. 2. The bait station 200 comprisesa housing 201 adapted to receive a bait matrix (not shown). The housing201 has openings 202 sized to permit termites to pass through to gainaccess to the bait matrix from a location outside the housing 201. Theoutside surface 203 of the housing 201 has a ridge 204 under eachopening 202. Without wishing to be bound by theory it is thought thatthe ridges may prevent soil from plugging the openings 202 when the baitstation 200 is installed in-ground. The housing 201 has a plate 205 atthe top and a tip 206 at the bottom. The tip 206 can be, for example, ametal tip installed over the end of a plastic housing 201, or the tip206 hard plastic that is part of the housing 201. The plate 205 may bestruck or pushed to install the bait station in-ground. The plate 205has an opening 207. A bait matrix can be installed in the housing 201through the opening 207.

Another bait station is shown in FIG. 3. The bait station 300 comprisesa housing 301 adapted to receive a bait matrix (not shown). The housing301 has openings 302 sized to permit termites to pass through to gainaccess to the bait matrix from a location outside the housing 301. Thehousing 301 has vertical ribs 303 to prevent the bait station 300 fromspinning after installation. Each rib 303 has a book 304 to prevent thebait station 300 from being too easily pulled out of the ground afterinstallation. The bait station 300 further comprises a removable lid305. The lid 305 comprises a handle 306 removably attached to a plate307. The plate has an insert 308 opposite the handle 306. The insert 308has a protruding notch 309 that fits into a groove 310 in the housing301. When the lid 305 is pushed onto the housing 301, the protrudingnotch 309 fits into the groove 310. The lid 305 is twisted so that theprotruding notch 309 rotates following the groove 310 to a lockingposition. The handle 306 is typically removed after the lid 305 isplaced on the housing.

FIGS. 4 a and 4 b show a bait station 400 according to an alternativeembodiment of this invention. The bait station 400 comprises a housing401 adapted to receive a bait matrix (not shown). The housing 401 hasopenings 402 sized to permit termites to pass through to gain access tothe bait matrix from a location outside die housing 401. The housing 401has threads 403 for allowing the bait station 400 to be screwed into theground. The housing 401 has a pointed tip 404 at the bottom and a flange405 at the top. The flange 405 is adapted to receive a removable handle406 (FIG. 4 a) that can be used to manually screw the bait station 400into the ground. After the bait station 400 is screwed into the ground,the handle 406 can be removed and replaced with a lid 407 (FIG. 4 b).

In an alternative embodiment of the invention, the bait matricesdescribed above can be used in bait stations that are known in the art.For example, suitable bait stations for use with the bait matrices aredisclosed in U.S. Pat. No. 5,695,776; U.S. Pat. No. 5,901,496; U.S. Pat.No. 5,921,018; U.S. Pat. No. 5,927,001; U.S. Pat. No. 5,937,571; U.S.Pat. No. 5,950,356; U.S. Pat. No. 5,899,018; U.S. Pat. No. 6,003,266;U.S. Pat. No. 6,016,625; U.S. Pat. No. 6,058,241; U.S. Pat. No.6,065,241; U.S. Pat. No. 6,071,529; and U.S. Pat. No. 6,079,150, all ofwhich are hereby incorporated by reference. Other suitable bait stationsfor use with the bait matrix of this invention are disclosed in WO00/19816.

Kits

This invention further relates to a kit for eliminating a termitecolony. The kit comprises:

i) a termiticidal bait matrix as described above, and

ii) a bait station as described above for containing the termiticidalbait matrix.

The kit may further comprise: iii) a nontermiticidal bait matrix, asdescribed above. The nontermiticidal bait matrix can be installed in thebait station and monitored to determine whether termites are present. Iftermites are detected, the termiticidal bait matrix can be installed inaddition to, or in place of, the nontermiticidal bait matrix.

The kit may further comprise: iv) a device for installing the baitstation in or near the locus of potential or known termite activity.When the device will be installed in-ground, a variety of devices may beused. Conventional devices, such as manual digging implements used toplant bulbs may be sufficient e.g., a spade, shovel, or manual auger canbe used. Other conventional devices such as an auger with an electricdrill can be used. Preferably, the device for installing a bait-stationin-ground creates a hole having about the same dimensions as the baitstation is used.

FIG. 5 shows a device 500 for installing a bait station in-ground. Thedevice 500 comprises a handle 501 that can be manually turned by aconsumer. The handle 501 is attached to a shaft 502 having a screw 503inside a pipe 504, with a blade 505 that is slightly larger in diameterthan the pipe 504 at the end of the screw 503. The pipe 504 allows soilto be pushed out of a hole bored by the blade 505 without disturbing thesoil surrounding the hole. The shaft 502 ends at a point 506 below theblade 505. A foot brace 507 is attached to the end of the pipe 504opposite the blade 505. The foot brace 507 can be used to exert downwardpressure as the handle 501 is twisted.

FIG. 6 a shows a device 600 for installing a bait station in-groundaccording to an alternative embodiment of this invention. The device 600is in its closed position. The device 600 comprises a sliding hammer 601that slides along a rod 604 having a top surface 606 and a bottomsurface 602. The sliding hammer 601 is used to strike the bottom surface602. Attached to the bottom surface 602 are a wedge (not shown) and atleast three wings 607 surrounding the wedge 605.

FIG. 6 b shows the device 600 in its open position. The sliding hammer601 drives the wedge 605 and wings 607 into the ground until the bottomsurface 602 reaches ground level 603. After the bottom surface 602reaches ground level 603, the top surface 606 is struck, for example,with a hammer or mallet 608, thereby driving the wedge 605 further intothe ground and spreading the wings 607.

FIG. 6 c shows a cross section of the device 600 in FIG. 6 a The device600 comprises a sliding hammer 601 that slides along a rod 604 having atop surface 606 and a bottom surface 602. The sliding hammer 601 is usedto strike the bottom surface 602. Attached to the surface are a wedge605 and at least three wings 607 surrounding the wedge 605. The wings607 are attached to the bottom surface 602 by hinges 609.

The kit may further comprise: v) a probe for determining whetherobstructions are present in-ground where the bait station will beinstalled. This probe can be, for example, a thin rod that can bemanually pushed into the ground where a consumer wishes to install abait station prior to pounding a bait station into the ground or boringa hole in which the bait station will be installed. The probe is used todetermine a suitable location for the bait station without boring alarge hole (i.e., large enough to hold the bait station). FIG. 7 shows aprobe for use in this invention. The probe 700 comprises a thin rod 701with a pointed tip 702 and a handle 703 for manually pressing the probe700 into the ground.

The kit may further-comprise: vi) information, instructions, or both,for using the kit. The information, instructions, or both, typicallycomprise words, pictures, videotapes, DVDs, or combinations thereofdescribing how to install and/or use the bait station, any optionaldevices for determining whether obstructions are present in-ground, andany optional devices for installing the bait station. Preferably, theinformation, instructions, or both, describe how to install and replacethe bait matrices (termiticidal and nontermiticidal, if any).

The bait stations in the kits of this invention can be installed in ornear a locus of potential or known termite activity. The locus may beabove-ground, in-ground, or on-ground In one embodiment of theinvention, the bait station can be installed by attaching it to astructure to be protected. In an alternative embodiment of theinvention, the bait station is installed in-ground by partially orcompletely burying the bait station, e.g., to control subterraneantermites. A bait station installed in-ground is typically buried in ahole about the same size and shape as the bait station. It is preferablenot to disturb the surrounding soil. Therefore, devices that bore holesabout the same size and shape as the bait station may be used forinstalling the bait stations.

FIG. 8 shows components of a kit 800 according to one embodiment of thisinvention. A bait station installation device 801 comprising a metalcomponent 802 having a point 803 at one end and a handle 804 at theother can be used to install a bait station 807. In one embodiment ofthe invention, the device 801 is used manually to bore a hole about thesame size and shape as the bait station 807. The device 801 can betapped into the ground using, for example, a hammer or mallet. Thedevice 801 is driven to a depth up to the flange 805 below the handle804. The device 801 may then be twisted to slightly loosen the soilfurther to make inserting the bait station 807 as effortless aspossible. After the hole is bored, the device 801 can be inserted intothe bait station 807 and used to tap or hammer the bait station into thehole. After the bait station 807 is installed in the hole, the device801 is removed and a bait matrix (not shown) can be inserted into thehousing 808 of the bait station 807. The housing 808 comprises aplurality of holes 809 sized to permit termites to gain entrance to thebait matrix from a location outside the housing 808. The bait station807 further comprises a pointed tip 810 at the bottom for insertion intothe hole and a top flange 811 for receiving the flange 805 of device801. The device 801 has a raised area 812 for indicating where to tapthe device with the hammer or mallet on top of the handle 804.

In an alternative embodiment of the invention, the device 801 isinstalled in the bait station 807 and the device 801 is struck with ahammer or mallet on the raised area 812 to install the bait station 807in the ground, without first boring a hole. In this embodiment, thepoint 803 of the device 801 directs the force from striking to the tip810 of the bait station 807. In this embodiment, the tip 810 ispreferably made of metal.

Methods of Use

This invention further relates to a method for eliminating a termitecolony comprising: I) installing in or near a locus of termite activityat least one bait station wherein the bait station contains atermiticidal bait matrix as described above. The number of bait stationsinstalled in step I) will depend on various factors such as the size ofthe structure to be protected. Typically, the bait station is installedabout 4 to about 6 feet from the structure to be protected. Typically,bait stations are installed about 10 to about 20 feet from each other ina perimeter around the structure. One skilled in the art would be ableto determine the appropriate number of bait stations without undueexperimentation.

The bait station can be installed by, for example, attaching it to astructure, placing it on the ground, or by burying it partially orcompletely in-ground.

The method for eliminating a termite colony may further comprise: II)adding more termiticidal bait matrix when the termiticidal bait matrixis exhausted or partially exhausted. Preferably, more termiticidal baitmatrix is added about every 1 to about every 3 weeks, more preferablyabout every 2 weeks until no termite activity is observed.

Using this method, a termite colony can be eliminated in about 3 monthsor less. Typically, the termite colony can be eliminated in about 3 toabout 6 weeks.

This invention further relates to a method for controlling termitescomprising:

I) monitoring for termite infestation by a process comprising

A) checking a bait station for signs of termites, wherein the baitstation is installed in or near a locus of potential termite activity,and wherein the bait station contains a nontermiticidal bait matrix, and

B) determining that termites are present; and

II) installing in the bait station a termiticidal bait matrix, asdescribed above.

Typically, the bait station is checked for signs of termites about every1 to about every 3 months. Signs of termites include the presence of mudtubes, discarded wings, or sawdust in or near the bait station.Typically, the nontermiticidal bait matrix is replaced about every 3 toabout every 9 months, more preferably about every 6 months, before it isdetermined that termites are present.

This method may further comprise: III) adding more termiticidal baitmatrix when the termiticidal bait matrix is exhausted or partiallyexhausted. Typically, more termiticidal bait matrix is added about every1 to about every 3 weeks, more preferably about every 2 weeks, until notermite activity is observed.

In a preferred embodiment of the invention, the termiticidal bait matrixcan be installed in the bait station without moving or disrupting thenontermiticidal bait matrix. Without wishing to be bound by theory, itis thought that moving or disrupting the nontermiticidal bait matrix maycause termites to abandon the bait station.

The bait stations in the termite control devices and kits of thisinvention can be installed in or near a locus of potential or knowntermite activity. In one embodiment of the invention, the bait stationcan be installed by attaching it to a structure to be protected. In apreferred embodiment of the invention, the device is buried, partiallyor completely in the ground, e.g., to control subterranean termites.“Structure to be protected” includes but is not limited to buildingssuch as dwellings and commercial buildings, as well as trees, shrubs,and telephone poles.

EXAMPLES

These examples are intended to illustrate the invention to those skilledin the art and should not be interpreted as limiting the scope of theinvention set forth in the claims.

Reference Example 1 Enzyme Activity on the Cellulase Complex Assay

This assay, which is used to measure enzyme activity on the cellulasecomplex employs, Cellazyme T tablets as the substrate. One-halfmilliliter aliquots of a properly diluted Trichoderma reesei cellulaseenzyme complex in sodium acetate buffer (25 millimolar pH adjusted to4.5) are equilibrated with 50 microliters of inhibitor solution (2.819milligrams/milliliter in dimethylsulfoxide, “DMSO”) at 40° C. for 5minutes in glass test tubes (16×120 millimeters). (In theenzyme-with-no-inhibitor test tubes, a 50 microliter aliquot of DMSO isadded to the test tubes in place of the 50 microliter of inhibitorsolution.) A Cellazyme T tablet is then added to the solution. Thetablet hydrates to form a suspension very rapidly, however thesuspension is not stirred. After 10 minutes at 40° C., 10 milliliters ofTrizma base solution (2% w/v, Sigma T-1503) are added before vigorousstirring on a vortex mixer to terminate the enzyme reaction. The slurryis allowed to stand at room temperature for 4–5 minutes, stirred again,and centrifuged at 2000 rpm for 10 minutes. The absorbance of thesupernatant is measured at 590 nanometers against a substrate/enzymeblank. (The substrate/enzyme blank is prepared and kept at roomtemperature by adding the Trizma base to the enzyme before addition ofthe substrate, thereby terminating the enzyme reaction before it canhydrolyze the substrate.)

Cellulase inhibition is measured as a reduction in rate of hydrolysis ofthe substrate, compared to the enzyme rate without inhibitor.

Reference Example 2 β-glucosidase Assay

This assay, which is used to measure enzyme activity for β-glucosidasesemploys p-nitrophenyl β-1,4-glucopyranoside (Sigma N-7006) as thesubstrate. A 1.7 millimolar substrate solution in MES buffer (0.05 MMES, pH 6.2) is used in the assay. $ glucosidase (Sigma G-4511) isdiluted in MES buffer to 0.05 milligrams/milliliter for use in theassay. The potential inhibitors for testing are contained in individualwells of a 96-well microtiter plate at 170 micromolar in DMSO. Somewells contain DMSO alone to serve as the no-inhibitor enzyme only wells.

Using a second 96-well microtiter plate, aliquots of the inhibitors (20microliters) and enzyme (25 microliters) are mixed with buffer (100microliters) and allowed to equilibrate at room temperature for 60minutes. Fifteen minutes into this equilibration, absorbance at 405nanometers is measured on the microtiter plate containing the mix toobtain blank values. A UV-visible spectrophotometer plate reader is usedto measure all 96-wells in one set.

When the 60-minute equilibration is complete, the substrate (25microliters) is added to each well, the enzyme reactions allowed to runfor 30 minutes, and then quenched with of 1 molar Na₂CO₃ solution (100microliters). Absorbance at 405 nanometers is measured on the reactionplate and the final absorbance calculated by subtracting thecorresponding blank value.

Cellulase inhibition is measured as a reduction in rate of hydrolysis ofthe substrate, compared to the enzyme rate without inhibitor.

Reference Example 3 Termiticide Activity

The tests, run in triplicate, are set up in three wells of a 6-wellpolystyrene microtiter plate (3 wells are empty). Four circles of either3.2 cm Whatman #1 filter paper (cellulose) for the food controls andtests on compounds, or 3.2 cm Whatman GF/C glass microfibre filters(borosilicate glass) for the no-food controls are stacked in each well.For the food controls, 600 microliters of water are added to each wen.For the no-food controls 1200 microliters of water are added to thosewells. If the test compounds are water soluble, they are added to thewells in 600 microliters of water to deliver 0.5% compound. Waterinsoluble test compounds are added to the wells in a solvent in whichthey are soluble to deliver 0.5% compound, the solvent is evaporated,and 600 microliters of water added per well.

Termiticidal activity for a compound is measured using termites from thegenus/specie Reticulitermes flavipes or Coptotermes formosanus. Thetermites are added in groups of 20 per well. Every 34 days the termitesare counted. The number of days until 50% death (DTD₅₀) and 100% deathfor termites is determined. These values for termites in the wellscontaining test compounds are compared to the food and no-food controlsto determine which compounds display termiticidal activity.

Reference Example 4 Termiticide Preference Test

The test is performed in 100 millimeter Petri dishes into which 20 gramsof washed sand and 2 milliliters of water are distributed. Six circles(three plain and thee treated with the compound of interest) of Whatman#1 filter paper (15 millimeters) are used as food. The treated circlesare prepared by pipetting a small volume (approximately 25 microliters)of a solution of the compound in an appropriate solvent onto the filterpaper circles, and then evaporating the solvent.

The filter paper circles are arranged in the dish in two rows using analternating pattern of treated and untreated. One hundred termites arethen added to the dish. The test is run for about 7 days to determine ifany eating preference is seen between the treated and untreated filterpaper circles.

Example 1 Determination of Termiticidal Activity

Various compounds were analyzed by the method of Reference Example 3.The results are in Table VI.

TABLE VI Compound/ CAS No. Structure DTD₅₀ Control Borosilicate Glass 5480-05-7

42 80-04-6

62 10192-62-8

70 154862-23-4

66 213193-76-1

60 50541-93-0

59

41 3363-56-2

63 195052-64-3

48%by day53 2226-96-2

32 1752-96-1

80 5437-98-9

31 10041-06-2

35 14090-83-6

35 28230-32-2

80 5613-46-7

42% atday 73 4199-10-4

77

33

27 Aspidosperma quebracho-blanco extract 23 Cucurbitaceae fruit & greentea extract 15 1464-44-4 Phenyl-beta-D-glycoside 15

Example 1 shows that various compounds can be used as termiticides inthis invention. Aspidosperma quebracho-blanco extract, Cucurbitaceaefruit & green tea extract, and Phenyl-beta-D-glycoside are suitable touse as the termiticides in this invention at levels reduced from that inExample 1.

Example 2 Determination of Preference

A compound having CAS No. 80-04-6 is tested according to the method ofReference Example 4, and the compound passes the preference test.

Example 3 Determination of Cellulase Inhibition

Various compounds are tested according to the method of ReferenceExample 1. Compounds exhibiting cellulase inhibition are shown in TableVII.

TABLE VII Compound/ CAS No. Structure 80-04-6

213193-76-1

50541-93-0

3363-56-2

10041-06-2

14090-83-6

28230-32-2

4199-10-4

Example 4 Determination of β-glucosidase Inhibition

Various compounds are tested according to the method of ReferenceExample 2. Compounds exhibiting β-glucosidase inhibition are shown inTable VIII.

TABLE VIII Compound/ CAS No. Structure 154862-23-4

213193-76-1

50541-93-0

3363-56-2

1950562-64-3

2226-96-2

1752-96-1

10041-06-2

14090-83-6

4199-10-4

Example 5 Synthesis of Compounds of Formula II

Compound I is synthesized in three steps from commercially availablepentabenzyl-glucopyranoside. The anomeric hydroxyl of i is firstoxidized to ii with the Dess-Martin Periodinane. The resulting lactoneis treated with Ruppert's reagent (trifluoromethyl-trimethylsilane) andcatalytic fluoride (TBAF) yielding a protected, cyclic hemi-ketal iii at70% yield. Subsequent treatment with Pearlman's catalyst (Palladiumhydroxide, 20% on carbon) provides the free sugar hemi-ketal I in 70%yield.

Compound II is obtained similarly. Perbenzylated cellobionolactone, iiais obtained as an intermediate by Br₂/water or electrochemical oxidationof cellobiose by methods known in the art; followed by benzylation. Oneskilled in the art would be able to synthesize compound II without undueexperimentation.

1. A cellulase inhibitor, comprising in combination: a) a compound offormula

wherein R¹ is selected from the group consisting of a bond, ahydrocarbon group, O, and NR¹⁴R¹⁵, wherein R¹⁴ and R¹⁵ are eachindependently selected from the group consisting of a hydrogen atom anda hydrocarbon group; each R² is independently selected from the groupconsisting of CH, a carbon atom, and a heteroatom; R³, R⁴, R⁵, and R⁶are each independently selected from the group consisting of hydrogenatoms, hydroxyl groups and methyl groups, and R⁷ and R⁸ are eachindependently selected from the group consisting of hydrogen atoms,oxygen atoms, nitrogen atoms, hydroxyl groups and NH₂ groups, wherein(i) when R⁷ is II, OH or NH₂, R⁹ is absent; and (ii) when R⁸ is II, OHor NH₂, R¹⁰ is absent; R⁹ and R¹⁰ are each independently selected fromthe group consisting of hydrocarbon groups of 1 to 6 carbon atoms, alkylesters of 1 to 6 carbon atoms, and amides; and R¹¹ and R¹² are eachindependently selected from the group consisting of saturated,unsaturated, and aromatic groups, b) a cellulose containing material,and c) water, wherein said cellulase inhibitor inhibits β-glucosidase,endoglucanase, exoglucanase or combinations thereof.
 2. The cellulaseinhibitor of claim 1, wherein the compound is selected from the groupconsisting of;

wherein Ac represents an acyl group.
 3. The cellulase inhibitor of claim1, wherein the cellulose containing material is selected from the groupconsisting of wood, charred wood, decayed wood, sawdust pulp, paper,cotton linter, cellulose ethers, and combinations thereof.
 4. Thecellulase inhibitor of claim 3, further comprising: d) one or moreoptional ingredients selected from the group consisting of attractants,defoamers, dextrin, dispersing agents, extenders, feeding stimulants,flavors, grain powder, moisture adjusting means, nitrogen sources,nutrients, penetrants, stabilizers, sugars, surface active agents,suspending agents, synergists, vegetable oils, and combinations thereof.5. A cellulase inhibitor comprising in combination: a) a termiticidecomprising a cellulase inhibitor, wherein the cellulase inhibitor isselected from the group consisting of: Bisphenol Λ diacetato, diammoniumhexachloropalladate, 2-amino-3-[(phenylmethyl)thio]-1 -propanol,bipenamol hydrochloride, compound having CAS Registry number213193-76-1,

combinations thereof, b) a cellulose containing material, and c) water.6. The cellulase inhibitor of claim 5, wherein the cellulase inhibitorinhibits β-glucosidase, endoglucanase, exoglucanase or a combinationsthereof.
 7. The cellulase inhibitor of claim 5, wherein the cellulosecontaining material is selected from the group consisting of wood,charred wood, decayed wood, sawdust, pulp, paper, cotton linter,cellulose ethers, and combinations thereof.
 8. The cellulase inhibitorof claim 5, further comprising: d) one or more optional ingredientsselected from the group consisting of attractants, defoamers, dextrin,dispersing agents, extenders, feeding stimulants, flavors, grain powder,moisture adjusting means, nitrogen sources, nutrients, penetrants,stabilizers, sugars, surface active agents, suspending agents,synergists, vegetable oils, and combinations thereof.
 9. A cellulaseinhibitor, comprising in combination: a) an imidazole compound selectedfrom the group consisting of:

b) a cellulose containing material, and c) water, wherein said compoundis used as a cellulase inhibitor.
 10. The ecliulase inhibitor of claim9, wherein the cellulase inhibitor inhibits β-glucosidase,endoglucanase, exoglucanase or combinations thereof.
 11. A cellulaseinhibitor comprising in combination: a) a compound selected from thegroup consisting of:

n-bromyl succinimide, 4,4-isoprophylidencdicyclohexanol,2,5,6-trimethylbenzylimidazole, hexachloropalladate compounds,polyphenols, and combinations thereof, b) a cellulosc containingmaterial, and c) water wherein said compound inhibits β-glucosidase,endoglucanase, exoglucanase or combinations thereof.